SnareSeq2

NOTE: Several versions of this metadata schema have been created over time. The (Latest) version contains most attributes, but there may be some deprecated attributes in the older versions for which data has been collected. SenNet is in the process of creating a reference which combines all of these versions into a single view. That reference will be available here once completed.

Version 1 (no longer accepting data)

Version 1 (no longer accepting data)

Attribute Type Description Allowable Values Required
version Allowable Value Version of the schema to use when validating this metadata. [‘1’] True
description Textfield Free-text description of this assay.   True
source_id Textfield SenNet Display ID of the source of the assayed tissue.   True
tissue_id Textfield SenNet Display ID of the assayed tissue.   True
execution_datetime Datetime Start date and time of assay, typically a date-time stamped folder generated by the acquisition instrument. YYYY-MM-DD hh:mm, where YYYY is the year, MM is the month with leading 0s, and DD is the day with leading 0s, hh is the hour with leading zeros, mm are the minutes with leading zeros.   True
protocols_io_doi Textfield DOI for protocols.io referring to the protocol for this assay.   True
operator Textfield Name of the person responsible for executing the assay.   True
operator_email Textfield Email address for the operator.   True
pi Textfield Name of the principal investigator responsible for the data.   True
pi_email Textfield Email address for the principal investigator.   True
assay_category Allowable Value Each assay is placed into one of the following 4 general categories: generation of images of microscopic entities, identification & quantitation of molecules by mass spectrometry, imaging mass spectrometry, and determination of nucleotide sequence. [‘sequence’] True
assay_type Allowable Value The specific type of assay being executed. [‘Slide-seq’] True
analyte_class Allowable Value Analytes are the target molecules being measured with the assay. [‘RNA’] True
is_targeted Allowable Value Specifies whether or not a specific molecule(s) is/are targeted for detection/measurement by the assay. [‘Yes’, ‘No’] True
acquisition_instrument_vendor Textfield An acquisition instrument is the device that contains the signal detection hardware and signal processing software. Assays generate signals such as light of various intensities or color or signals representing the molecular mass.   True
acquisition_instrument_model Textfield Manufacturers of an acquisition instrument may offer various versions (models) of that instrument with different features or sensitivities. Differences in features or sensitivities may be relevant to processing or interpretation of the data.   True
rnaseq_assay_method Textfield The kit used for the RNA sequencing assay   True
library_construction_protocols_io_doi Textfield A link to the protocol document containing the library construction method (including version) that was used, e.g. “Smart-Seq2”, “Drop-Seq”, “10X v3”.   True
library_layout Allowable Value State whether the library was generated for single-end or paired end sequencing. [‘single-end’, ‘paired-end’] True
library_adapter_sequence Textfield Adapter sequence to be used for adapter trimming   True
puck_id Textfield Slide-seq captures RNA sequence data on spatially barcoded arrays of beads. Beads are fixed to a slide in a region shaped like a round puck. Each puck has a unique puck_id.   True
is_technical_replicate Allowable Value Is the sequencing reaction run in repliucate, TRUE or FALSE [‘Yes’, ‘No’] True
bead_barcode_read Textfield Which read file contains the bead barcode   True
bead_barcode_offset Textfield Position(s) in the read at which the bead barcode starts   True
bead_barcode_size Textfield Length of the bead barcode in base pairs   True
library_pcr_cycles Numeric Number of PCR cycles to amplify cDNA   True
library_pcr_cycles_for_sample_index Numeric Number of PCR cycles performed for library indexing   True
library_final_yield_value Numeric Total number of ng of library after final pcr amplification step. This is the concentration (ng/ul) * volume (ul)   True
library_final_yield_unit Allowable Value Units of final library yield [‘ng’] False
library_average_fragment_size Numeric Average size in basepairs (bp) of sequencing library fragments estimated via gel electrophoresis or bioanalyzer/tapestation.   True
sequencing_reagent_kit Textfield Reagent kit used for sequencing   True
sequencing_read_format Textfield Slash-delimited list of the number of sequencing cycles for, for example, Read1, i7 index, i5 index, and Read2.   True
sequencing_read_percent_q30 Numeric Q30 is the weighted average of all the reads (e.g. # bases UMI * q30 UMI + # bases R2 * q30 R2 + …)   True
sequencing_phix_percent Numeric Percent PhiX loaded to the run   True
contributors_path Textfield Relative path to file with ORCID IDs for contributors for this dataset.   True
data_path Textfield Relative path to file or directory with instrument data. Downstream processing will depend on filename extension conventions.   True
Version 0

Version 0

Attribute Type Description Allowable Values Required
source_id Textfield SenNet Display ID of the source of the assayed tissue.   True
tissue_id Textfield SenNet Display ID of the assayed tissue.   True
execution_datetime Datetime Start date and time of assay, typically a date-time stamped folder generated by the acquisition instrument. YYYY-MM-DD hh:mm, where YYYY is the year, MM is the month with leading 0s, and DD is the day with leading 0s, hh is the hour with leading zeros, mm are the minutes with leading zeros.   True
protocols_io_doi Textfield DOI for protocols.io referring to the protocol for this assay.   True
operator Textfield Name of the person responsible for executing the assay.   True
operator_email Textfield Email address for the operator.   True
pi Textfield Name of the principal investigator responsible for the data.   True
pi_email Textfield Email address for the principal investigator.   True
assay_category Allowable Value Each assay is placed into one of the following 4 general categories: generation of images of microscopic entities, identification & quantitation of molecules by mass spectrometry, imaging mass spectrometry, and determination of nucleotide sequence. [‘sequence’] True
assay_type Allowable Value The specific type of assay being executed. [‘Slide-seq’] True
analyte_class Allowable Value Analytes are the target molecules being measured with the assay. [‘RNA’] True
is_targeted Allowable Value Specifies whether or not a specific molecule(s) is/are targeted for detection/measurement by the assay. [‘Yes’, ‘No’] True
acquisition_instrument_vendor Textfield An acquisition instrument is the device that contains the signal detection hardware and signal processing software. Assays generate signals such as light of various intensities or color or signals representing the molecular mass.   True
acquisition_instrument_model Textfield Manufacturers of an acquisition instrument may offer various versions (models) of that instrument with different features or sensitivities. Differences in features or sensitivities may be relevant to processing or interpretation of the data.   True
rnaseq_assay_method Textfield The kit used for the RNA sequencing assay   True
library_construction_protocols_io_doi Textfield A link to the protocol document containing the library construction method (including version) that was used, e.g. “Smart-Seq2”, “Drop-Seq”, “10X v3”.   True
library_layout Allowable Value State whether the library was generated for single-end or paired end sequencing. [‘single-end’, ‘paired-end’] True
library_adapter_sequence Textfield Adapter sequence to be used for adapter trimming   True
puck_id Textfield Slide-seq captures RNA sequence data on spatially barcoded arrays of beads. Beads are fixed to a slide in a region shaped like a round puck. Each puck has a unique puck_id.   True
is_technical_replicate Allowable Value Is the sequencing reaction run in repliucate, TRUE or FALSE [‘Yes’, ‘No’] True
bead_barcode_read Textfield Which read file contains the bead barcode   True
bead_barcode_offset Textfield Position(s) in the read at which the bead barcode starts   True
bead_barcode_size Textfield Length of the bead barcode in base pairs   True
library_pcr_cycles Numeric Number of PCR cycles to amplify cDNA   True
library_pcr_cycles_for_sample_index Numeric Number of PCR cycles performed for library indexing   True
library_final_yield_value Numeric Total number of ng of library after final pcr amplification step. This is the concentration (ng/ul) * volume (ul)   True
library_final_yield_unit Allowable Value Units of final library yield [‘ng’] False
library_average_fragment_size Numeric Average size in basepairs (bp) of sequencing library fragments estimated via gel electrophoresis or bioanalyzer/tapestation.   True
sequencing_reagent_kit Textfield Reagent kit used for sequencing   True
sequencing_read_format Textfield Slash-delimited list of the number of sequencing cycles for, for example, Read1, i7 index, i5 index, and Read2.   True
sequencing_read_percent_q30 Numeric Q30 is the weighted average of all the reads (e.g. # bases UMI * q30 UMI + # bases R2 * q30 R2 + …)   True
sequencing_phix_percent Numeric Percent PhiX loaded to the run   True
contributors_path Textfield Relative path to file with ORCID IDs for contributors for this dataset.   True
data_path Textfield Relative path to file or directory with instrument data. Downstream processing will depend on filename extension conventions.   True